“Age-related osteoblast dysfunction is the main cause of age-related bone loss.

Trans-caryophyllene (TC) is an important constituent of the essential oils derived from several species of medicinal plants.

In this study, we investigated the effects of TC on osteoblast function in osteoblastic MC3T3-E1 cells. The results indicate that TC caused a significant elevation in collagen content, alkaline phosphatase activity, osteocalcin production, and mineralization, which are the four markers that account for the various stages of osteoblastic differentiation.

Our findings that TC promotes the formation of a mineralized extracellular matrix help to elucidate the role of CB2 signaling in the formation of bone and the maintenance of normal bone mass.”

https://www.ncbi.nlm.nih.gov/pubmed/28026135

“Trans-caryophyllene is a sesquiterpene present in many medicinal plants’ essential oils, such as Ocimum gratissimum and Cannabis sativa.”  https://www.ncbi.nlm.nih.gov/pubmed/24055516