“Cannabinoids are secondary natural products from the plant Cannabis sativa L.
Therapeutic indications of cannabinoids currently comprise a significant area of medicinal research.
We have expressed the Δ9-tetrahydrocannabinolic acid synthase (THCAS) and cannabidiolic acid synthase (CBDAS) recombinantly in Komagataella phaffii and could detect eight different products with a cannabinoid scaffold after conversion of the precursor cannabigerolic acid (CBGA).
Besides five products remaining to be identified, both enzymes were forming three major cannabinoids of C. sativa – Δ9-tetrahydrocannabinolic acid (THCA), cannabidiolic acid (CBDA) and cannabichromenic acid (CBCA).
These studies lay the groundwork for further research as well as biotechnological cannabinoid production.”